Topic outline
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General
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FLUORESCENCE SPECTROSCOPY
HighlightedLECTURES 11 to 13 from the 28th of march to april 6th 2018
What is molecular fluorescence emission.
Jablonsky diagram: singlet and triplet electronic molecular states. Fluorescence/phosphorescence.
Radiative and non-radiative decay channels.
Absorption spectra/ emission spectra: Stokes shift.
Electric dipole transitions. Franck-Condon Principle.
Fluorescence Quantum yields and lifetimes.
Fluorescence polarization.
Continuous distributions of lifetimes (Alcala1987).
Forster Resonance Energy Transfer (FRET)
Scheme of a basic fluorimeter (T configuration).
Introduction to the FRAP technique: the FRAP recovery function (follow PBC chap. 13.1 and 13.2.3)*.
Proteins intrinsic fluorophores: Green fluorescent proteins.
Scheme of a Fluorescence Microscopy. Confocal microscope, tomography. Two-photons spectroscopy.
Study Materials: Chapter 1 from J. R. Lackowicz, Principles of Fluorescence Spectroscopy, Springer 2010. See also: D. Jameson, Introduction to Fluorescence, CRC press 2014 chapp. 1, 2 and 3.
*This topic is optional.