OBJECTIVES

The new generation of sequencing technologies has provided unforeseen chances for high-throughput functional genomic studies. These technologies have been applied in a variety of contexts, including whole-genome sequencing, discovery of transcription factor binding sites, mapping out the DNA accessibility and RNA expression profiling. Intriguingly, recent annotation efforts focused on the discovery of novel noncoding RNA genes and regulatory elements that control temporal or spatial gene expression along cell differentiation. The course of Molecular Biology and Genomics is designed to provide students with an introduction to the structure and function of genomes and transcripts in humans and in other model organisms. Topics discussed will include modern genome sequencing technologies, as well the recent in silico and in vivo approaches used for functional genomics and for the functional role of emerging non-coding RNA classes (practical examples taken from recent literature will be used). The course also provides students with basic knowledge for accessing browsers and public databases for the analysis of gene expression data, GO and miRNA target prediction software.

By the end of the course, students will be able to apply the acquired knowledge to the study of the basic mechanisms of gene expression, as well as of complex processes such as development, cell division and differentiation, and to exploit them for a practical use in both basic and applied research.

The course includes 48 hours of theoretical lectures, computer exercises and student seminars.