The purpose of this course is to provide the students with the fundamental intellectual tools required to carry out measurements, analyses and interpretation of enzyme kinetics. At the end of the course, the students will be confident with the elementary concepts of chemical kinetics, will understand the theoretical basis of steady state and rapid equilibrium enzyme kinetics, and will be able to derive the relevant rate equations. They will also be familiar with the practical aspects of enzyme kinetics, such as enzyme assay methods and the use of computer software to analyse kinetics data. The understanding and analytical skills of the students will include enzyme reactions with more than one substrate, enzyme inhibition and activation. Concerning the transient phase of enzyme reactions, students will understand the theoretical basis of rapid kinetics and the main experimental techniques used for their measurement; they will also be able to analyse and interpret rapid kinetics.
- Teacher: Roberto Contestabile